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In the field of genetic engineering, the construction of expression vectors utilizing the potent promoters of polycomb proteins within the structural genes of baculoviruses yields efficient and even high-level expression of numerous target genes. Thus, baculovirus-insect cell expression systems are emerging as powerful tools for protein production. Due to the diverse factors influencing protein expression, the GMP manufacturing process is essential to guarantee protein quality and activity for scientific research or production purposes. Currently, GMP-grade proteins have become ubiquitous in vaccine and drug development, proteomics research, and gene therapy.
Creative BioMart is able to provide professional protein expression services, utilizing a baculovirus-insect cell system that operates under GMP standards to deliver high-quality, efficient GMP-grade proteins.
Due to compliance with strict regulations, GMP proteins are produced for use in drug development, in vitro diagnostics, and gene therapy.
Insect cells have post-translational modification mechanisms similar to those of mammalian cells, such as glycosylation and phosphorylation, which allow the expressed proteins to have biological activities similar to those of natural proteins.
The baculovirus is incapable of infecting vertebrates, and the viral promoter does not work in mammalian cells. This system demonstrates superior reliability and efficacy compared to other systems when used to express toxic proteins or oncogenes.
The system is capable of expressing as much as 50% of the entire protein content in insect cells and can concurrently attain high levels of multiple exogenous genes. Soluble and stable expression can also be realized using vector modification.
Repeatable amplification for large-scale production of biologically active recombinant proteins.
Can be used in the research of antibody-virus vaccines, oncogene protein, gene therapy, and other fields.
Figure 1. GMP-grade baculovirus-insect cell protein expression process.
To enhance the expression level of the specific protein in insect cells, it is necessary to optimize the gene sequence linked to the intended protein.
The optimized target gene is inserted into a baculovirus expression vector to form a recombinant virus.
The target gene is transfected into insect cells for amplification using techniques such as phosphate co-precipitation, electroporation, and viral transfection. The titer of the recombinant baculovirus particles is measured by cytopathic effect (CPE) or ELISA.
To obtain highly expressed proteins, expression efficiency can be improved by optimizing culture conditions and selecting appropriate promoters and signal peptides.
Purification techniques such as ion exchange and molecular sieve chromatography can be utilized to separate the target proteins from insect cells and remove impurities.
The quality and purity of target proteins are tested using biological assays such as SDS-PAGE, ELISA, and Western blot.
Creative BioMart is committed to providing GMP-level protein expression services to facilitate research in biomedical fields such as drug development, vaccine preparation, and gene therapy. If you are interested in our services, please contact us.
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