GMP-Grade Yeast Protein Expression Service

GMP proteins are used as key tools in the fields of antibody production, disease model construction, and drug screening. With increasing demands for protein quality and purity in various research areas, the emergence of a GMP-grade yeast protein expression system offers a solution to this problem. Using yeast as the expression host, exogenous genes are introduced into yeast cells and expressed and modified accordingly under ideal conditions to obtain excellent GMP proteins. Therefore, the system is deemed to be a proficient method of generating GMP proteins on a large-scale industrial level. Additionally, the protein production process adheres to GMP requirements and enforces strict quality control measures.

Creative BioMart has comprehensive and professional GMP-grade yeast protein expression services and a strict quality control system, which can meet the experimental demand for large-scale GMP proteins.

Why Choose GMP-Grade Yeast Protein Expression System?

• Comply with GMP Requirements

Due to compliance with strict regulations, GMP proteins are produced for use in life sciences, in vitro diagnostics, and biopharmaceuticals.

• High Expression

Since yeast cells have a high multiplication rate and gene expression capacity, large quantities of target proteins can be produced in a short period of time.

• Simple Cultivation

Yeast is a unicellular microorganism that grows and reproduces quickly while requiring low culture conditions, making it easy to culture on a large scale.

• High Safety

The expression system does not present a concern of exogenous gene contamination, resulting in a low risk to ensure experimental safety.

• Powerful Post-Translational Modification Function

Due to the process of glycosylation and phosphorylation, the GMP proteins attained exhibit increased biological activity.

• Large-Scale Production

The large production volume can meet the demand for GMP protein in the bio-industry and pharmaceutical fields.

Figure 1. GMP-grade yeast protein expression process.

Key Steps for Our Service

• Optimize Codons and Synthesis Gene

Due to the concatenation of codons, codon optimization is carried out in gene synthesis by replacing rare codons and optimizing the mRNA structure to improve protein expression and prevent non-expression or low expression of proteins.

• Expression Vector Construction

Recombinant plasmids are constructed by ligating target genes with appropriate expression vectors. Expression vectors usually include functional elements such as promoters, transcription termination sequences, and selection tags.

• Transformation and Expression

Introduce the constructed expression vector into yeast cells to create a genetic fusion between the vector and the yeast cells.

• Screening of Target Strains

The transformed yeast cells are screened for recombinant strains carrying the target gene using selection markers.

• Protein Purification

Purification of expressed target proteins can be performed using techniques such as affinity chromatography, gel filtration, and ion exchange chromatography.

• QC

Our Outstanding Advantages

• One-stop service platform. Our services cover a series of processes from gene design to protein expression, and our advanced technology and rich experience can meet the different customization needs of our customers.
• GMP-grade. Our services adhere to strict GMP standards to ensure compliance with regulatory requirements and to deliver protein products of high quality and purity.
• Customization. Tailor-made solutions according to customer's needs, delivering the ideal high-quality GMP proteins.
• Strict regulatory compliance. The production of recombinant proteins complies with GMP regulations and standards to ensure product safety and compliance.

Creative BioMart possesses professional biotechnology and quality management capabilities, pledging to offer research institutes and biological entities worldwide GMP proteins of high purity and activity. If you are interested in our services, please contact us.