GMP-Grade Mammalian Cell Protein Expression Service

In the field of biopharmaceuticals, the production of both target protein drugs and antibody drugs requires high stability, good purity, and uniform quality proteins. Therefore, it is essential to determine the best way to express and produce such proteins effectively. Proteins manufactured in accordance with GMP standards by utilizing mammalian cell lines like CHO cells and HEK293 cells exhibit enhanced quality and activity, catering to the evolving research requirements. Due to the extensive research and development in biomedicine, the system can produce GMP recombinant proteins for the treatment of various diseases such as diabetes and blood disorders.

Creative BioMart provides a comprehensive solution for transient transfection expression, recombinant antibody expression, and stable cell line construction using GMP equipment and proprietary high-expression vectors to produce GMP-grade proteins from milligrams to grams in a short time.

Why Choose GMP-Grade Mammalian Cell Protein Expression System?

• Comply with GMP Requirements

Due to compliance with strict regulations, GMP proteins are produced for use in life sciences, in vitro diagnostics, and biopharmaceuticals.

• Products Closer to the Natural State

Post-translational modifications like glycosylation, phosphorylation, and methylation lead to the formation of proteins that have greater biological activity and stability.

• Strong Folding and Assembly Ability

Provide a suitable environment to promote the correct folding and assembly of proteins, which is conducive to the study and application of protein structure and function.

• Conform to Complex Protein Structure

Suitable for the expression of larger and more complex proteins, especially those containing multiple structural domains or cumulants. Since mammalian cells are more capable of translating and folding complex proteins, they are better able to meet the requirements of their expression and function.

• Large-scale Production

The technology is very mature, and the high expression of target proteins allows for large-scale production while favoring lower production costs and higher yields.

• High Safety

Proteins produced using this system are relatively safe due to their resistance to viral and bacterial infections. The high degree of glycosylation and homogeneity of the product proteins can effectively improve the efficacy of the drug and reduce the side effects of the drug.

Figure 1. GMP-grade mammalian cell protein expression process.

Key Steps for Our Service

• Optimize Codons and Synthesis Gene

According to the protein sequence to be expressed, codons are optimized to avoid problems such as mutations, deletions, or insertions, and the target gene is designed and synthesized.

• Select Host Cell

Currently, commonly used host cells include CHO, HEK293, COS, etc.

• Expression Vector Construction

To introduce the target gene into the host cell, it is necessary to select the appropriate promoter, selector, and transcription terminator when constructing the expression vector.

• Cell Transient Transfection

Calcium chloride, phosphate, DEAE-glucan, PEI, and electroporation.

• Screening of Expression Cell Lines

Generally, the expression cell line is selected by antibiotic screening and fluorescence screening.

• Expression and Purification

After expression, the target protein is purified by affinity chromatography, ion exchange chromatography, and gel filtration chromatography to remove impurities and contaminants.

• Identification and Detection

Protein gel electrophoresis, Western blot, mass spectrometry, and other biotechnologies.

• Large-scale Production
• QC

Our Outstanding Advantages

• GMP-grade specifications. Our services adhere to strict GMP standards to ensure compliance with regulatory requirements and to deliver protein products of high quality and purity.
• One-stop service platform. Our range of research projects span from gene composition to protein synthesis. Our innovative technology and extensive knowledge allow us to fulfill a variety of tailored requests from our clientele.
• Professional technical support. Our team has extensive expertise and experience in providing technical support and solutions to customers.

Creative BioMart provides a full range of services and technical support to help customers achieve safe, efficient, and large-scale GMP-grade protein expression. If you have any questions or service requests, please feel free to contact us to get a reply the first time.