GMP-Grade E. coli Protein Expression Service

The E. coli expression system is one of the earlier adopted technologically mature protein expression systems. However, protein expression is susceptible to interference by various factors, resulting in changes in properties and parameters, and ultimately in reduced quality and activity. To ensure high-quality proteins, it is crucial to follow a GMP production process. GMP-grade E. coli protein expression is a secure, efficient, and economical protein production technique widely employed in biopharmaceuticals and other fields. By strictly controlling the operating environment and quality standards during the production process, it ensures that GMP proteins can be produced consistently and stably for the intended use and requirements.

Creative BioMart offers GMP-grade E. coli protein expression services with a rigorous quality control system. Our one-stop service platform features rapid and efficient production to meet the demands of large-scale GMP protein.

Why Choose GMP-Grade E. coli Protein Expression System?

• Comply with GMP Requirements

The manufacturing process strictly conforms to regulations, and GMP proteins find applications in biomedicine, antibody preparation, immunotherapy, and allied fields.

• Clear Genetic Background

E. coli, as the microorganism used to express exogenous proteins, has been subjected to full gene sequencing, and open reading frames, which facilitates the design and organization of experiments.

• Simple Culture

The rapid reproduction of E. coli makes it convenient to culture and operate. Additionally, the stable genetic characteristics of E. coli reduce its susceptibility to mutation, which facilitates subsequent experimental procedures.

• Easy to Large-scale Production

The genetic background, growth and reproduction, and metabolic regulation of E. coli have been extensively studied, making it a suitable expression system for industrialized production.

• High Activity and Stability of Products

E. coli can express biologically active proteins, and its expressed proteins have good stability under various conditions.

Figure 1. GMP-grade E. coli protein expression process.

Key Steps for Our Service

• Design and Synthesis Gene

According to needs, optimize codons and gene sequences and synthesize target genes.

• Expression Vector Construction

Recombinant plasmids are constructed by ligating target genes with appropriate expression vectors. Expression vectors usually include functional elements such as promoters, transcription termination sequences, and selection tags.

• Transformation and Expression

The expression vector was transferred into E. coli to enable the target gene to be successfully expressed in E. coli. Induce small-scale expression, evaluate initial expression levels, and optimize expression conditions.

• Identification

Detect proteins through biological methods such as ELISA, Western blot, SDS-PAGE, and BCA protein concentration determination.

• Protein Purification

Purification of expressed target proteins can be performed using techniques such as affinity chromatography, gel filtration, and ion exchange chromatography.

• QC

Finally, the biological activity, molecular weight, stability, and other indicators of the protein are examined.

Our Outstanding Advantages

• Large-scale GMP production. With a highly automated production process, a strict quality control system, and a professional technical team, we can provide large-scale and reliable GMP protein expression service.
• Abundant host bacteria and vector libraries. According to the different needs of customers, the diversified selection of vectors and strains can optimize the protein expression process more flexibly to obtain high-quality and high-activity proteins.
• One-stop service platform. Covering the entire process from gene design to protein expression, we can deliver the target protein directly.

Creative BioMart is equipped with large-scale GMP-grade protein production capacity to meet the needs of research or industrial production projects. Through our one-stop platform, our research team will provide you with a full range of technical support and services, while delivering high-quality protein products that meet GMP standards. Please feel free to contact us for more details about our services.