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| Cat#: | Spike-038THP |
| Product Name: | Recombinant SARS-CoV-2 Spike Protein(RBD), His-tag, GMP Grade |
| similar: | Spike |
| Alias: | Spike; RBD |
| Source: | HEK293 |
| Species: | SARS-CoV-2 |
| Tag : | His |
| Protein length: | Arg319-phe541 |
| Product Overview: | Recombinant SARS-CoV-2 Spike Protein(RBD) (Arg319-phe541) was fused with His tag, produced in HEK293 cell in an animal component free process under cGMP guidelines. |
| Description: | The single-positive-strand RNA genome of SARS-CoV-2, encodes four major structural proteins: spike (S), envelope (E), membrane (M) and nucleocapsid (N). The spike protein comprises an N-terminal (S1) subunit, which contains the roughly 200-residue receptor binding domain (RBD), and a C-terminal subunit (S2), which contains the fusion protein. The RBD of SARS-CoV-2 binds more tightly to the extracellular domain of angiotensin-converting enzyme 2 (ACE2) than the homologous SARS-CoV (2002) RBD. The higher affinity results from sequence changes in RBD and this has been proposed to underlie the higher transmissibility of SARS-CoV-2. The previously determined structures showed that SARS-CoV RBD contains a core structure and a receptor-binding motif (RBM) and that the RBM binds to the outer surface of the claw-like structure of ACE2. Importantly, two virus-binding hot spots on human ACE2 has been identified. A number of naturally selected RBM mutations occurred near these two virus-binding hot spots, and these residues largely determined the host range of SARS-CoV. |
| Form: | Phosphate Buffered Saline, 5%mannital, 0.05% Tween20, pH7.4 |
| Molecular Mass: | 26.20 kDa |
| Endotoxin: | >95% pure (SDS-PAGE). |
| Storage: | For long term storage, the product should be stored at -20°C or lower. |
| Reconstitution: | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
| Common Name: | S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ] |
| Official Symbol: | S |
| GeneID: | 43740568 |
| Protein Refseq: | YP_009724390.1 |
| UniProt ID: | P0DTC2 |
Not For Human Consumption!
