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| Cat#: | IGF-08THP |
| Common Name: | IGF |
| Product Name: | GMP Recombinant Human LR3 Insulin-like Growth Factor-1 |
| Product Overview: | GMP Recombinant Human LR3 Insulin-like Growth Factor-1 was expressed in E. coli. |
| Description: | Long R3 IGF-1 (LR3 IGF-1) is a 9.2 kDa synthetic analog of IGF-1 that is generated by modifying the aa sequence for mature human IGF-1. These modifications include the substitution of an Arg for Glu at position 3 of the mature IGF-1 sequence and the addition of a thirteen aa N-terminal extension, which is derived from methionyl porcine Growth Hormone. These aa changes generate a protein that is still capable of binding to IGF-1 and Insulin receptors, but shows considerably lower affinity binding to IGFBPs compared to wild-type IGF-1. As a result, LR3 IGF-1 has an increased halflife and displays increased biological potency compared to IGF-1. |
| Source: | E. coli |
| Species: | Human |
| Form: | Lyophilized from a 0.22 µm filtered concentrated solution in PBS pH 7.0. |
| Bio-activity: | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using FDC-P1 cells is ≤ 2.0 ng/ml, corresponding to a specific activity of > 1.0. |
| Molecular Mass: | Approximately 9.2 kDa, a single non-glycosylated polypeptide chain containing 83 amino acids. |
| Protein length: | 83 aa |
| AA Sequence: | MFPAMPLSSL FVNGPRTLCG AELVDALQFV CGDRGFYFNK PTGYGSSSRR APQTGIVDEC CFRSCDLRRL EMYCAPLKPA KSA |
| Endotoxin: | Less than 0.01 EU per μg by the LAL method. |
| Purity: | > 95 % by SDS-PAGE and HPLC analyses. |
| Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Reconstitution: | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Gene Name: | IGF1 insulin like growth factor 1 [ Homo sapiens (human) ] |
| Official Symbol: | IGF1 |
| Synonyms: | IGF; MGF; IGFI; IGF-I |
| GeneID: | 3479 |
| mRNA Refseq: | NM_000618 |
| Protein Refseq: | NP_000609 |
| MIM: | 147440 |
| UniProt ID: | P05019 |
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Not For Human Consumption!
