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| Cat#: | IGF-08THP |
| Common Name: | IGF |
| Product Name: | GMP Recombinant Human LR3 Insulin-like Growth Factor-1 |
| Product Overview: | GMP Recombinant Human LR3 Insulin-like Growth Factor-1 was expressed in E. coli. |
| Description: | Long R3 IGF-1 (LR3 IGF-1) is a 9.2 kDa synthetic analog of IGF-1 that is generated by modifying the aa sequence for mature human IGF-1. These modifications include the substitution of an Arg for Glu at position 3 of the mature IGF-1 sequence and the addition of a thirteen aa N-terminal extension, which is derived from methionyl porcine Growth Hormone. These aa changes generate a protein that is still capable of binding to IGF-1 and Insulin receptors, but shows considerably lower affinity binding to IGFBPs compared to wild-type IGF-1. As a result, LR3 IGF-1 has an increased halflife and displays increased biological potency compared to IGF-1. |
| Source: | E. coli |
| Species: | Human |
| Form: | Lyophilized from a 0.22 µm filtered concentrated solution in PBS pH 7.0. |
| Bio-activity: | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using FDC-P1 cells is ≤ 2.0 ng/ml, corresponding to a specific activity of > 1.0. |
| Molecular Mass: | Approximately 9.2 kDa, a single non-glycosylated polypeptide chain containing 83 amino acids. |
| Protein length: | 83 aa |
| AA Sequence: | MFPAMPLSSL FVNGPRTLCG AELVDALQFV CGDRGFYFNK PTGYGSSSRR APQTGIVDEC CFRSCDLRRL EMYCAPLKPA KSA |
| Endotoxin: | Less than 0.01 EU per μg by the LAL method. |
| Purity: | > 95 % by SDS-PAGE and HPLC analyses. |
| Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Reconstitution: | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Gene Name: | IGF1 insulin like growth factor 1 [ Homo sapiens (human) ] |
| Official Symbol: | IGF1 |
| Synonyms: | IGF; MGF; IGFI; IGF-I |
| GeneID: | 3479 |
| mRNA Refseq: | NM_000618 |
| Protein Refseq: | NP_000609 |
| MIM: | 147440 |
| UniProt ID: | P05019 |
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For research use only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
