GMP Recombinant S. pyogenes CRISPR-Cas9 Protein

Cat#:	Cas9-109THP
Common Name:	Cas9
Product Name:	GMP Recombinant S. pyogenes CRISPR-Cas9 Protein
Product Overview:	GMP Recombinant S. pyogenes CRISPR-Cas9 Protein(Q99ZW2)(Asp2-Asp1368) is expressed from E.coli for genome editing applications with CRISPR technology.
Source:	E. coli
Species:	S. pyogenes
Form:	Supplied as 0.22 μm filtered solution in 30 mM Tris-HCl, 0.3 M NaCl, 50% Glycerol, 0.1 mM EDTA, pH 7.4. Please dilute to the desired concentration according to the concentration of the solution shown on the product label.
Bio-activity:	Cas9 cuts the substrate DNA standard through the in vitro cutting experiment. The result of the figure shows that the cleavage activity of Kactus Cas9 is equivalent to that of other brands.
Molecular Mass:	The protein has a predicted MW of 163 kDa based on Tris-Bis PAGE result.
Protein length:	Asp2-Asp1368
Endotoxin:	Less than 0.01 EU per μg by the LAL method.
Purity:	> 95% as determined by Tris-Bis PAGE > 95% as determined by HPLC
Storage:	Valid for 12 months from date of receipt when stored at -20°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
Concentration:	10 mg/ml
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