Recombinant Human CSF3 protein GMP Grade(NP_000750) was expressed in E. coli.
Source:
E. coli
Species:
Human
Form:
Lyophilized from a 0.2 μm filtered concentrated solution in 10 mM sodium acetate buffer, containing 5% trehalose, pH 4.0.
Bio-activity:
The ED50 determined by a cell proliferation assay using murine NFS-60 cells is less than 0.1 ng/mL, corresponding to a specific activity of >1.0 × 10^7 IU/mg.
Molecular Mass:
Approximately 18.7 kDa, a single non-glycosylated polypeptide chain containing 174 amino acids.
This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably des centigradecated. Upon reconsitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw centigrade centigradeles.
Reconstitution:
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Not For Human Consumption!
