Recombinant Human EPCAM protein, His-tag, GMP Grade
Product Overview:
Recombinant Human EPCAM protein GMP Grade(NP_002345.2)(241 aa) was expressed in HEK293 cells with a C-terminal His Tag.
Source:
HEK293 Cells
Species:
Human
Tag :
His
Form:
Freeze-dried powder contains Sterile PBS (pH 7.4) with 6% mannitol.
Bio-activity:
The determination is based on the ability of the immobilized protein to support the adhesion of the L cell-mouse fibroblast cell line. When 5×104 cells/well were added to Recombinant Human EpCAM and human fibronectin coated plates (0.5ug/ml), cell adhesion was enhanced in a dose-dependent manner. The ED50 of this effect is 1-2.4ug/ml.
Molecular Mass:
Human EpCAM Protein has a predicted MW of 29.1 kDa.
Protein length:
241 aa
Endotoxin:
Less than 0.1EU/ug by the LAL method.
Purity:
Greater than 95% by SDS-PAGE and HPLC
Storage:
The lyophilized preparation can be stored at 4°C for 24 months, and the dissolved liquid can be stored at -20°C for 6-12 months to avoid repeated freezing and thawing
Reconstitution:
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Not For Human Consumption!
