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| Cat#: | IL12-128THP |
| Common Name: | IL12 |
| Product Name: | Recombinant Human IL12 protein, GMP Grade |
| Product Overview: | Recombinant Human IL12 protein GMP Grade(NP_002178) was expressed in Insect Cells. |
| Source: | Insect Cells |
| Species: | Human |
| Form: | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Bio-activity: | Measured in a cell proliferation assay using PHA-stimulated human T lymphoblasts. The ED50 for this effect is 0.01-0.05 ng/mL. The specific activity of rHuIL-12 is approximately 1.1 × 104 units/μg, which is calibrated against rHuIL-12 WHO Standard (NIBSC code: 95/544). |
| Molecular Mass: | Apparent molecular mass of 60 kDa in SDS-PAGE under non-reducing conditions, 41 kDa and 29 kDa under reducing conditions, a disulfide-linked heterodimer consisting of the 306 amino acid rHuIL-12 p40 and the 197 amino acid rHuIL-12 p35 subunits. |
| Protein length: | HuIL-12 p40: Ile23 - Ser328; Accession # P29460 HuIL-12 p35: Arg23 - Ser219; Accession # P29459 |
| Endotoxin: | Less than 1.0 EU per 1 μg determined by the LAL method. |
| Purity: | Greater than 97%, by SDS-PAGE with silver staining. |
| Storage: | Avoid repeated freeze-thaw cycles. 12 months, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Reconstitution: | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
| Gene Name: | IL12A interleukin 12A [ Homo sapiens (human) ] |
| Official Symbol: | IL12A |
| Synonyms: | P35; CLMF; NFSK; NKSF1; IL-12A |
| GeneID: | 3592 |
| mRNA Refseq: | NM_000882.4 |
| Protein Refseq: | NP_000873.2 |
| UniProt ID: | P29459 |
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Not For Human Consumption!
