Recombinant Human IL12 protein, His-tag, GMP Grade
- Specification
- Related Products
| Cat#: |
IL12-160THP |
| Common Name: |
IL12 |
| Product Name: |
Recombinant Human IL12 protein, His-tag, GMP Grade |
| Product Overview: |
Recombinant Human IL12 protein GMP Grade(NP_000873),fused to His tag, was expressed in HEK293 Cells. |
| Source: |
HEK293 Cells |
| Species: |
Human |
| Tag : |
His |
| Form: |
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5 % mannitol and 0.01% Tween80. |
| Bio-activity: |
Measured in a cell proliferation assay using anti-CD3 antibody activated human peripheral blood mononuclear cell (PBMC). The ED50 for this effect is typically 0.4-2 ng/ml. The specific activity of recombinant human IL-12 is approximately 1.3 x 10^4 units/μg, which is calibrated against recombinant human IL-12 WHO Standard (NIBSC code: 95/544). |
| Molecular Mass: |
The GMP recombinant IL12 heterodimer of human IL12A/IL12B comprises 525 (208 + 317) amino acids and has a calculated molecular mass of 60.2 (24 + 36.2) kDa. |
| Endotoxin: |
Less than 0.01 EU per μg determined by the LAL method. |
| Purity: |
Greater than 95 % as determined by SDS-PAGE. |
| Storage: |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution: |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
| Gene Name: |
IL12A interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35) [ Homo sapiens ] |
| Official Symbol: |
IL12A |
| Synonyms: |
IL12A; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); NKSF1; interleukin-12 subunit alpha; CLMF; cytotoxic lymphocyte maturation factor 1; p35; IL 12; subunit p35; IL 12A; IL35 subunit; interleukin 12; interleukin 12 alpha chain; natural killer cell stimulatory factor 1; 35 kD subunit; NF cell stimulatory factor chain 1; NFSK; CLMF p35; IL-12 subunit p35; IL-12, subunit p35; interleukin 12, p35; interleukin-12 alpha chain; NK cell stimulatory factor chain 1; cytotoxic lymphocyte maturation factor 1, p35; cytotoxic lymphocyte maturation factor 35 kDa subunit; natural killer cell stimulatory factor 1, 35 kD subunit; P35; CLMF; CLMF2; IL-12A; IL-12B; IL12; IL12 p70; IMD28; IMD29; NFSK; NKSF; NKSF2; P35 |
| GeneID: |
3592 |
| mRNA Refseq: |
NM_000882 |
| Protein Refseq: |
NP_000873 |
| MIM: |
161560 |
| UniProt ID: |
P29459 |
Not For Human Consumption!
