Recombinant Human IL6 protein, GMP Grade
- Specification
- Related Products
| Cat#: |
IL6-210THP |
| Common Name: |
IL6 |
| Product Name: |
Recombinant Human IL6 protein, GMP Grade |
| Product Overview: |
Recombinant Human IL6 protein GMP Grade(NP_000591) was expressed in E. coli. |
| Source: |
E. coli |
| Species: |
Human |
| Form: |
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5 % mannitol and 0.01% Tween80. |
| Bio-activity: |
Measured by its binding ability in a functional ELISA. Immobilized human IL6 at 10μg/mL (100μL/well) can bind human IL6R-His, the EC50 of human IL6R-His is 0.1-0.5 μg/mL. Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.2-0.8 ng/mL. The specific activity of recombinant human IL-6 is approximately 3.2 x 10^5 IU/μg, which is calibrated against human IL-6 WHO International Standard (NIBSC code: 89/548). |
| Molecular Mass: |
The GMP recombinant human IL6 consists of 184 amino acids and has a calculated molecular mass of 20.9 kDa. |
| Endotoxin: |
Less than 0.01 EU per μg determined by the LAL method. |
| Purity: |
Greater than 95 % as determined by SDS-PAGE. |
| Storage: |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution: |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
| Gene Name: |
IL6 interleukin 6 (interferon, beta 2) [ Homo sapiens ] |
| Official Symbol: |
IL6 |
| Synonyms: |
IL6; interleukin 6 (interferon, beta 2); IFNB2; interleukin-6; BSF2; HGF; HSF; IL 6; CDF; BSF-2; IFN-beta-2; interferon beta-2; interleukin BSF-2; hybridoma growth factor; CTL differentiation factor; B-cell stimulatory factor 2; B-cell differentiation factor; IL-6; |
| GeneID: |
3569 |
| mRNA Refseq: |
NM_000600 |
| Protein Refseq: |
NP_000591 |
| MIM: |
147620 |
| UniProt ID: |
P05231 |
For research use only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
