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| Cat# | Product Name | Availability | Size | Price | Qty |
|---|---|---|---|---|---|
| TNF-106THP | Recombinant Human TNF protein, GMP Grade | 10ug | $998 |
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| Quote Order Bulk Order | |||||
| Cat#: | TNF-106THP |
| Common Name: | TNF |
| Product Name: | Recombinant Human TNF protein, GMP Grade |
| Product Overview: | Recombinant Human TNF protein GMP Grade(NP_000585) was expressed in E. coli. |
| Source: | E. coli |
| Species: | Human |
| Form: | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Bio-activity: | Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 25-100 pg/mL. The specific activity of recombinant human TNF-alpha GMP is approximately 7.6 x 10^4 IU/μg, which is calibrated against human TNF-alpha WHO International Standard (NIBSC code: 88/786). |
| Molecular Mass: | Predicted Molecular Mass: 17 kDa SDS-PAGE: 17 kDa, reducing conditions |
| Endotoxin: | Less than 0.10 EU per 1 μg determined by the LAL method. |
| Purity: | Greater than 97%, by SDS-PAGE with silver staining. |
| Storage: | Avoid repeated freeze-thaw cycles. 12 months, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Reconstitution: | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
| Gene Name: | TNF tumor necrosis factor [ Homo sapiens ] |
| Official Symbol: | TNF |
| Synonyms: | TNF; tumor necrosis factor; DIF; TNF superfamily; member 2; TNF alpha; TNFSF2; TNF-a; cachectin; tumor necrosis factor-alpha; TNFA; TNF-alpha; |
| GeneID: | 7124 |
| mRNA Refseq: | NM_000594 |
| Protein Refseq: | NP_000585 |
| MIM: | 191160 |
| UniProt ID: | P01375 |
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Not For Human Consumption!
