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| Cat# | Product Name | Availability | Size | Price | Qty |
|---|---|---|---|---|---|
| TNF-106THP | Recombinant Human TNF protein, GMP Grade | 10ug | $998 |
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| Quote Order Request a Bulk Order | |||||
| Cat#: | TNF-106THP |
| Common Name: | TNF |
| Product Name: | Recombinant Human TNF protein, GMP Grade |
| Product Overview: | Recombinant Human TNF protein GMP Grade(NP_000585) was expressed in E. coli. |
| Source: | E. coli |
| Species: | Human |
| Form: | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Bio-activity: | Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 25-100 pg/mL. The specific activity of recombinant human TNF-alpha GMP is approximately 7.6 x 10^4 IU/μg, which is calibrated against human TNF-alpha WHO International Standard (NIBSC code: 88/786). |
| Molecular Mass: | Predicted Molecular Mass: 17 kDa SDS-PAGE: 17 kDa, reducing conditions |
| Endotoxin: | Less than 0.10 EU per 1 μg determined by the LAL method. |
| Purity: | Greater than 97%, by SDS-PAGE with silver staining. |
| Storage: | Avoid repeated freeze-thaw cycles. 12 months, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Reconstitution: | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
| Gene Name: | TNF tumor necrosis factor [ Homo sapiens ] |
| Official Symbol: | TNF |
| Synonyms: | TNF; tumor necrosis factor; DIF; TNF superfamily; member 2; TNF alpha; TNFSF2; TNF-a; cachectin; tumor necrosis factor-alpha; TNFA; TNF-alpha; |
| GeneID: | 7124 |
| mRNA Refseq: | NM_000594 |
| Protein Refseq: | NP_000585 |
| MIM: | 191160 |
| UniProt ID: | P01375 |
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For research use only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
